Description
Available at 2, 3, and 5 um particle sizes and ranging in length from 150 to 500 mm, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates. By reducing the particle size from 5µm to 2µm, the resolution between aggregates and monomers was greatly improved. Furthermore, by reducing the column length from 300mm to 150mm using a 2µm particle, 50% less run time was achieved, while maintaining resolution as compared to the original method that used a 5µm, 4.6 × 300mm column.